Composite

Part:BBa_M36558

Designed by: Ashley Henderson   Group: Stanford BIOE44 - S11   (2014-10-24)


PoPS estrogen receptor beta and ERE operon.

This composite part is designed to code for the Estrogen Receptor Beta and has a promoter and operon at the end that is designed to arrest transcription of whatever gene follows the promoter at the end of our composite part when estrogen is detected. The parts that make up the composite are the promoter, which is a strong, general E. coli promoter, a medium strength ribosome binding site, the cDNA for estrogen receptor beta, the stop codon, the general terminator which arrests transcription, the strong, general E. coli promoter for the gene that follows ER beta, and the ERE operon that ER beta can bind to to arrest transcription of the gene that follows the ER beta gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 84
    Illegal PstI site found at 1228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 84
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1734
    Illegal NheI site found at 1757
    Illegal PstI site found at 1228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 84
    Illegal BglII site found at 1178
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 84
    Illegal PstI site found at 1228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 84
    Illegal PstI site found at 1228
    Illegal NgoMIV site found at 204
    Illegal AgeI site found at 1576
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None